Gargle Nucleic Acid Reagents Extraction Reagents
KA'IDAR GANO
Samfurin ya ƙunshi gargle, cakuda nanospheres superparamagnetic, da na musamman na lysis reagents.Keɓaɓɓen bead ɗin maganadisu na musamman suna da alaƙa mai kyau ga kayan aikin jiki (ciki har da ƙwayoyin cuta kyauta da ƙwayoyin cuta masu kamuwa da ƙwayoyin cuta).Lokacin da ake tuntuɓar maganin lysis, ƙwayoyin da ba na ionic cell / nucleus-membrane-breaking surfactants da protease inhibitors a cikin maganin zai iya hana ayyukan DNA / RNA enzyme, da kuma daidaita acid nucleic.Dukkan abubuwan acid nucleic a cikin sassan jiki na gargle ana fitar da su cikin inganci cikin maganin lysis, da sauri suna samun acid nucleic.Samfuran gargle da ke amfani da wannan kit ɗin baya buƙatar tsarkakewar acid nucleic, wanda zai iya shafa kai tsaye ga kayan gano acid nucleic na ƙasa.
Babban abubuwan da ke cikin reagent
An nuna abubuwan da aka haɗa a cikin tebur 1.
Tebur 1 Abubuwan da ake Lodawa da Loading a Kit
Sunan sashi | Manyan abubuwan da aka gyara | Girma (1) | Girma (10) | Girma (30) | Girma (50) |
1. Gari A | NaCl | 8ml/tubu | 8ml/tube *10 tubes | 8ml/tube *30 tubes | 8ml/tube *50 tubes |
2. Mai Tarin Gargle | PP | guda 1 | 10 inji mai kwakwalwa | 30 inji mai kwakwalwa | 50 inji mai kwakwalwa |
3. Maganin Ingantawa B | Magnetic beads | 2 ml / tube | 2ml/tube *10 tubes | 2ml/tube *30 tubes | 2ml/tube *50 tubes |
4 Lysis Buffer C | Protease K | 0.2ml/ guda | 0.2ml/ guda * 10 inji mai kwakwalwa | 0.2ml/ guda * 30 inji mai kwakwalwa | 0.2ml/ guda * 50 inji mai kwakwalwa |
5. Magnetic Cap | Magnet | guda 1 | 10 inji mai kwakwalwa | 30 inji mai kwakwalwa | 50 inji mai kwakwalwa |
Abubuwan da aka gyara suna aiki na tsawon watanni 12.
Abubuwan da ake buƙata a cikin hakar acid nucleic, amma ba a haɗa su cikin kit ɗin ba:
1. Abubuwan amfani: 1.5ml EP tube;
2. Kayan aiki: Ruwan wanka (ko wanka na ƙarfe), pipettes, da centrifuge.
Bayanan asali
Samfuran bukatun:
1. Mai dacewa da hakar acid nucleic da haɓaka samfuran gargle.
2. Za a ƙara samfurin gargle zuwa maganin haɓaka B a cikin lokaci bayan tattarawa.Za'a canza bead ɗin maganadisu zuwa ma'aunin lysis na C nan da nan.Za'a iya adana samfuran da aka ƙara zuwa ma'aunin lysis C a zafin jiki.
Bayanin tattarawa: 1 yanki / akwati, 10 inji mai kwakwalwa / akwatin, 30 inji mai kwakwalwa / akwatin, da 50 inji mai kwakwalwa / akwati.
Yanayin ajiya: The enrichment bayani B da lysis bayani C ya kamata a adana for 12 watanni a 2-8 ℃, da sauran aka gyara za a iya adana for 12 months karkashin r dakin zafin jiki;Ana iya jigilar kayan kuma a adana shi na ɗan lokaci a ƙarƙashin yanayin zafi, wanda ba zai wuce kwanaki 5 ba.
Lokacin aiki: watanni 12
Takaddun shaidar rikodin na'urar likitanci No./Buƙatun fasaha A'a.:HJXB No. 20220086.
Ranar amincewa da sake duba Umarnin:
Ranar amincewa: Oktoba 26, 2022