Kit ɗin Haɗin Acid Nucleic (A02)
Ka'idar ganowa
Bayan fitar da kwayar halittar DNA ta hanyar rarrabuwar sel tare da ma'aunin lysis, dutsen maganadisu zai iya zaɓan ɗaure DNA ɗin genomic a cikin samfurin.Za'a iya cire ƙananan ƙazantattun ƙazanta waɗanda ƙwanƙolin maganadisu ke ɗauka ta wurin buffer ɗin wanki.A cikin TE, ƙwanƙwasa maganadisu na iya sakin DNA mai iyaka, samun ingantaccen DNA na genome.Wannan hanya mai sauƙi ce kuma mai sauri kuma ingancin DNA da aka fitar yana da girma, wanda zai iya biyan buƙatu don gano DNA methylation.A halin yanzu, kayan hakar da ke kan ƙwanƙolin maganadisu na iya dacewa da haƙar acid nucleic ta atomatik, tare da haɗuwa da manyan ayyukan hakar nucleic acid.
Babban abubuwan da ke cikin reagent
An nuna abubuwan da aka gyara a cikin tebur 1:
Tebur 1 Abubuwan Reagent da Loading
| Sunan sashi | Manyan abubuwan da aka gyara | Girma (48) | Girma (200) |
| 1. Digestion Buffer A | Tris, SDS | 15.8 ml / kwalban | 66ml/kwalba |
| 2. Lysis Buffer L | Guanidinium Isothiocyanate, Tris | 15.8 ml / kwalban | 66ml/kwalba |
| 3. Wanke Buffer A | NaCl, Tris | 11 ml / kwalba | 44 ml / kwalba |
| 4. Wanke Buffer B | NaCl, Tris | 13 ml/kwali | 26.5ml/kwalba *2 |
| 5. TE | Tris, EDTA | 12 ml/kwali | 44 ml / kwalba |
| 6. Protease K maganin | Protease K | 1.1ml/ guda | 4.4ml/ guda |
| 7. Magnetic bead suspension 2 | Magnetic beads | 0.5ml/ guda | 2.2ml/ guda |
| 8. Umurnai don fitar da reagents nucleic acid | / | 1 kwafi | 1 kwafi |
Abubuwan da ake buƙata a cikin hakar acid nucleic, amma ba a haɗa su cikin kit ɗin ba:
1. Reagent: Anhydrous ethanol, isopropanol, da PBS;
2. Abubuwan amfani: 50mL centrifuge tube da 1.5mL EP tube;
3. Kayan aiki: Ruwa mai wanka, pipettes, magnetic shelf, centrifuge, 96-riji farantin (atomatik), kayan aikin cirewa na nucleic acid atomatik (atomatik).
Bayanan asali
Samfuran bukatun:
1.A ganowa za a kammala a karkashin 7-day ajiya na yanayi zafin jiki bayan tarin na mahaifa exfoliated cell samfurin (wanda ba kayyade).
2. An gama gano ganowa a karkashin ajiya na kwanaki 30 na yanayi na yanayin yanayi mai tasowa bayan tarin samfurin tantanin halitta (gyarawa) ..
3.Za a kammala ganowa a ƙarƙashin ajiya na kwanaki 30 na yanayin zafi bayan tattara samfurin fitsari;Za a kammala ganowa a cikin lokaci bayan tattara samfuran ƙwayoyin halitta na al'ada.
Bayanin yin kiliya:200 inji mai kwakwalwa / akwatin, 48 inji mai kwakwalwa / akwati.
Yanayin ajiya:2-30 ℃
Lokacin aiki:watanni 12
Na'urar da ta dace:Tianlong NP968-C nucleic acid hakar kayan aiki, Tiangen TGuide S96 nucleic acid hakar kayan aiki, GENE DIAN EB-1000 nucleic acid hakar kayan aiki.
Takaddun shaidar rikodin na'urar likitanci No./Buƙatun fasaha A'a.:HJXB No. 20210100.
Ranar amincewa da sake duba umarnin:Ranar amincewa: Nuwamba 18, 2021
Game da Mu
A matsayin babban kamfani na fasaha wanda aka kafa a cikin 2018 ta manyan ƙwararrun epigenetic, Epiprobe yana mai da hankali kan gano ƙwayoyin ƙwayoyin cuta na cutar kansa DNA methylation da madaidaicin masana'antar theranostics.Tare da tushen fasaha mai zurfi, muna nufin jagorantar zamanin sabbin samfura don ƙaddamar da ciwon daji a cikin toho!
Dangane da binciken Epiprobe core na dogon lokaci, haɓakawa da canji a fagen DNA methylation tare da sabbin sabbin abubuwa, haɗe tare da keɓance maƙasudin DNA methylation na cututtukan daji, muna amfani da na musamman multivariate algorithm hada manyan bayanai da fasahar fasaha ta wucin gadi zuwa da kansa ya haɓaka keɓantaccen fasaha mai kariya na ruwa mai ƙima.Ta hanyar nazarin matakin methylation na takamaiman rukunin rukunin DNA na ɓarke a cikin samfurin, ana guje wa gazawar hanyoyin gwaji na al'ada da iyakancewar aikin tiyata da huda, wanda ba wai kawai gano ainihin cututtukan daji na farko ba, har ma yana ba da damar saka idanu na lokaci-lokaci. na faruwar ciwon daji da ci gaban ci gaba.
