TAGMe DNA Methylation Kits (qPCR) don Ciwon daji na Urothelial
SIFFOFIN KIRKI
Daidaitawa
Ingantattun samfuran asibiti sama da 3500 a cikin binciken cibiyar makafi sau biyu, samfurin yana da ƙayyadaddun 92.7% da azanci na 82.1%.
Dace
Ana iya kammala ainihin fasahar gano Me-qPCR methylation a mataki ɗaya cikin sa'o'i 3 ba tare da canjin bisulfite ba.
Mara cin zali
Ana buƙatar 30 ml na samfurin fitsari kawai don gano nau'ikan ciwon daji guda 3, gami da kansar ƙashin ƙashin ƙugu, ciwon fitsari, ciwon daji na mafitsara a lokaci guda.
Yanayin aikace-aikace
Ganewar Mahimmanci
Yawan mutanen da ke fama da hematuria mara radadi/ da ake zargin suna da ciwon urothelial (cancer na urethra / ciwon ƙashin ƙugu)
Gwajin Hadarin Ciwon daji
Tiyata / chemotherapy-bukatar yawan jama'a tare da urothelial carcinoma;
Sa ido Maimaituwa
Yawan jama'ar bayan tiyata tare da carcinoma urothelial
AMFANI DA NUFIN
Ana amfani da wannan kit ɗin don gano in vitro qualitative gano hypermethylation na Urothelial Carcinoma (UC) gene a cikin urothelial samfurori.Kyakkyawan sakamako yana nuna haɓakar haɗarin UC, wanda ke buƙatar ƙarin cystoscope da / ko gwajin histopathological.Akasin haka, sakamakon gwaji mara kyau yana nuna cewa haɗarin UC yana da ƙasa, amma haɗarin ba za a iya cire shi gaba ɗaya ba.Ya kamata ganewar asali na ƙarshe ya dogara ne akan cystoscope da / ko sakamakon histopathological.
KA'IDAR GANO
Wannan kit ɗin ya ƙunshi reagent na hakar acid nucleic da PCR reagent.Ana fitar da acid nucleic ta hanyar tushen dutsen maganadisu.Wannan kit ɗin ya dogara ne akan ka'idar hanyar PCR mai kyalli, ta amfani da methylation-takamaiman PCR na ainihin lokacin don nazarin DNA samfuri, kuma a lokaci guda gano wuraren CpG na UC gene da alamar ingancin sarrafawa ta ciki gutsutsutsu G1 da G2.Matsayin methylation na UC gene, wanda ake kira da ƙimar Ni, ana ƙididdige shi bisa ga ƙimar UC gene methylated DNA amplification Ct da ƙimar Ct na tunani.Halin UC gene hypermethylation tabbatacce ko mara kyau an ƙaddara bisa ga ƙimar Ni.
DNA Methylation Kits (qPCR) don Ciwon daji na Urothelial
| Aikace-aikacen asibiti | Binciken asibiti na asibiti na urothelial cancer;kimanta ingancin aikin tiyata/chemotheray;saka idanu maimaituwa bayan tiyata |
| Gane kwayoyin halitta | UC |
| Nau'in samfurin | Samfurin exfoliated tantanin fitsari (lamin fitsari) |
| Hanyar gwaji | Fluorescence ƙididdige fasahar PCR |
| Samfura masu dacewa | Farashin ABI7500 |
| Bayanin tattarawa | 48 gwaje-gwaje/kit |
| Yanayin Ajiya | Kit A ya kamata a adana a 2-30 ℃ Ya kamata a adana Kit B a -20± 5℃ Yana aiki har zuwa watanni 12. |
