TAGMe DNA Methylation Kits (qPCR) don Ciwon daji na Cervical / Endometrial Cancer
SIFFOFIN KIRKI
Mara cin zali
Ana amfani da buroshin mahaifa da samfuran smear Pap.
Dace
Ana iya kammala ainihin fasahar gano Me-qPCR methylation a mataki ɗaya cikin sa'o'i 3 ba tare da canjin bisulfite ba.
Da wuri
Ana iya ganowa a matakin farko na ciwon daji.
Kayan aiki da kai
Tare da keɓantaccen software na bincike na sakamako, fassarar sakamakon an sarrafa ta atomatik kuma ana iya karantawa kai tsaye.
Yanayin aikace-aikace
Farkon nunawa
Mutane masu lafiya
Gwajin Hadarin Ciwon daji
Yawan jama'a masu haɗari (tabbatacce ga babban haɗarin ɗan adam papillomavirus (hrHPV) ko tabbatacce ga cytology exfoliation na mahaifa / tabbatacce ga babban haɗarin ɗan adam papillomavirus (hrHPV) ko tabbatacce ga cytology exfoliation na mahaifa)
Sa ido Maimaituwa
Yawan tsinkaya
AMFANI DA NUFIN
Ana amfani da wannan kit ɗin don gano ingancin in vitro na hypermethylation na gene PCDHGB7 a cikin samfuran mahaifa.Don ciwon daji na mahaifa, sakamako mai kyau yana nuna haɗarin haɗari na digiri na 2 ko mafi girma / ƙarin ci gaba na intraepithelial neoplasia na mahaifa (CIN2 +, ciki har da CIN2, CIN3, adenocarcinoma a wuri, da ciwon daji na mahaifa), wanda ke buƙatar ƙarin bincike na colposcopy da / ko histopathological jarrabawa. .Akasin haka, sakamakon gwaji mara kyau yana nuna cewa haɗarin CIN2+ ya yi ƙasa kaɗan, amma haɗarin ba za a iya cire shi gaba ɗaya ba.Ya kamata ganewar asali na ƙarshe ya dogara ne akan colposcopy da/ko sakamakon histopathological.Bugu da ƙari, don ciwon daji na endometrial, sakamako mai kyau yana nuna ƙarin haɗarin cututtukan cututtuka na endometrial precancerous da ciwon daji, wanda ke buƙatar ƙarin nazarin tarihin tarihin endometrium.Akasin haka, sakamakon gwaje-gwaje mara kyau ya nuna cewa haɗarin cututtukan ciwon daji na endometrial da ciwon daji yana da ƙasa, amma ba za a iya cire haɗarin gaba ɗaya ba.Binciken ƙarshe ya kamata ya dogara ne akan sakamakon binciken histopathological na endometrium.
PCDHGB7 memba ne na dangin protocadherin γ tarin kwayoyin halitta.An samo Protocadherin don tsara tsarin tafiyar da ilimin halitta kamar yaduwar kwayar halitta, sake zagayowar tantanin halitta, apoptosis, mamayewa, ƙaura da autophagy na ƙwayoyin tumor ta hanyar hanyoyi daban-daban na sigina, da kuma yin shiru da kwayar cutar ta hanyar hypermethylation na yankin mai gabatarwa yana da alaka da abin da ya faru da ci gaba. na ciwon daji da yawa.An bayar da rahoton cewa hypermethylation na PCDHGB7 yana da alaƙa da ciwace-ciwace iri-iri, irin su lymphoma ba Hodgkin, ciwon nono, ciwon mahaifa, ciwon daji na endometrial da ciwon daji na mafitsara.
KA'IDAR GANO
Wannan kit ɗin ya ƙunshi reagent na hakar acid nucleic da PCR reagent.Ana fitar da acid nucleic ta hanyar tushen dutsen maganadisu.Wannan kit ɗin ya dogara ne akan ƙa'idar hanyar PCR mai ƙididdigewa, ta amfani da methylation-takamaiman halayen PCR na ainihin lokacin don nazarin DNA samfuri, kuma a lokaci guda gano wuraren CpG na PCDHGB7 gene da alamar ingancin sarrafawa ta ciki gutsuttsura kwayoyin G1 da G2.An ƙididdige matakin methylation na PCDHGB7 a cikin samfurin, ko ƙimar Me, bisa ga PCDHGB7 gene methylated DNA amplification Ct da ƙimar Ct na tunani.PCDHGB7 gene hypermethylation tabbatacce ko korau matsayi an ƙaddara bisa ga ƙimar Ni.
