TAGMe DNA Methylation Kits (qPCR) don Ciwon daji na Endometrial
SIFFOFIN KIRKI
Daidaitawa
Ingantattun samfuran asibiti sama da 800 a cikin binciken cibiyar makafi biyu, samfurin yana da ƙayyadaddun 82.81% da ƙwarewar 80.65%.
Dace
Ana iya kammala ainihin fasahar gano Me-qPCR methylation a mataki ɗaya cikin sa'o'i 3 ba tare da canjin bisulfite ba.
Da wuri
Ana iya ganowa a matakin farko na ciwon daji.
Kayan aiki da kai
Ana amfani da buroshin mahaifa da samfuran smear Pap.
AMFANI DA NUFIN
Ana amfani da wannan kit ɗin don gano ingancin in vitro na hypermethylation na kwayar halittar PCDHGB7 na mahaifa.Sakamakon sakamako mai kyau yana nuna haɗarin ciwon daji na endometrial precancerous raunuka da ciwon daji, wanda ke buƙatar ƙarin bincike na histopathological na endometrium.Akasin haka, sakamakon gwaje-gwaje mara kyau ya nuna cewa haɗarin cututtukan ciwon daji na endometrial da ciwon daji yana da ƙasa, amma ba za a iya cire haɗarin gaba ɗaya ba.Binciken ƙarshe ya kamata ya dogara ne akan sakamakon binciken histopathological na endometrium.PCDHGB7 memba ne na dangin protocadherin γ tarin kwayoyin halitta.An samo Protocadherin don tsara tsarin tafiyar da ilimin halitta kamar yaduwar kwayar halitta, sake zagayowar tantanin halitta, apoptosis, mamayewa, ƙaura da autophagy na ƙwayoyin tumor ta hanyar hanyoyi daban-daban na sigina, da kuma yin shiru da kwayar cutar ta hanyar hypermethylation na yankin mai gabatarwa yana da alaka da abin da ya faru da ci gaba. na ciwon daji da yawa.An bayar da rahoton cewa hypermethylation na PCDHGB7 yana da alaƙa da ciwace-ciwace iri-iri, irin su lymphoma ba Hodgkin, ciwon nono, ciwon mahaifa, ciwon daji na endometrial da ciwon daji na mafitsara.
KA'IDAR GANO
Wannan kit ɗin ya ƙunshi reagent na hakar acid nucleic da PCR reagent.Ana fitar da acid nucleic ta hanyar tushen dutsen maganadisu.Wannan kit ɗin ya dogara ne akan ƙa'idar hanyar PCR mai ƙididdigewa, ta amfani da methylation-takamaiman halayen PCR na ainihin lokacin don nazarin DNA samfuri, kuma a lokaci guda gano wuraren CpG na PCDHGB7 gene da alamar ingancin sarrafawa ta ciki gutsuttsura kwayoyin G1 da G2.An ƙididdige matakin methylation na PCDHGB7 a cikin samfurin, ko ƙimar Me, bisa ga PCDHGB7 gene methylated DNA amplification Ct da ƙimar Ct na tunani.PCDHGB7 gene hypermethylation tabbatacce ko korau matsayi an ƙaddara bisa ga ƙimar Ni.
Yanayin aikace-aikace
Farkon nunawa
Mutane masu lafiya
Gwajin Hadarin Ciwon daji
Ƙungiyoyi masu haɗari (mutanen da ke da zubar da jini na al'ada bayan menopause, kauri na endometrial, da dai sauransu).
Sa ido Maimaituwa
Yawan tsinkaya
Muhimmancin asibiti
Binciken farko don yawan jama'a masu lafiya:Ciwon daji na endometrial da raunukan da aka rigaya za a iya tantance su daidai;
Ƙimar haɗari ga yawan masu haɗari:Za a iya yin kima mai haɗari ga mutanen da ke da zubar da jini na al'ada na al'ada da kuma kauri na endometrial bayan menopause don taimakawa wajen ganewar asibiti;
Sa ido kan maimaita yawan jama'a:Ana iya yin sa ido kan sake dawowar jama'a bayan tiyata don hana jinkirin jiyya da ke haifar da sake dawowa.
Tarin samfurin
Hanyar Samfur: Sanya abin da za'a iya zubar da mahaifa a cikin mahaifa os, a hankali shafa goshin mahaifa kuma a juya sau 4-5 a agogo, a hankali cire goshin mahaifa, saka shi cikin maganin adana tantanin halitta, sannan a sanya masa alama don jarrabawar ta gaba.
Ajiye samfurori:Ana iya adana samfurori a cikin zafin jiki har zuwa kwanaki 14, a 2-8 ℃ har zuwa watanni 2, kuma a -20± 5 ℃ har zuwa watanni 24.
Tsarin Ganewa: Sa'o'i 3 (Ba tare da tsari na hannu ba)
TAGMe DNA Methylation Kits (qPCR) don Ciwon daji na Endometrial
| Aikace-aikacen asibiti | Binciken asibiti na asibiti na ciwon daji na endometrial |
| Gane kwayoyin halitta | PCDHGB7 |
| Nau'in samfurin | Samfuran mahaifar mata |
| Hanyar gwaji | Fluorescence ƙididdige fasahar PCR |
| Samfura masu dacewa | Farashin ABI7500 |
| Bayanin tattarawa | 48 gwaje-gwaje/kit |
| Yanayin Ajiya | Ya kamata a adana Kit A a 2-30 ℃ Ya kamata a adana Kit B a -20± 5℃ Yana aiki har zuwa watanni 12. |
Game da Mu
Epiprobe yana da cikakken kayan aikin gine-gine: Cibiyar samar da GMP tana rufe yanki na murabba'in murabba'in 2200, kuma tana kula da tsarin gudanarwa mai inganci na ISO13485, wanda ya dace da buƙatun samarwa na kowane nau'in samfuran reagent na gwajin kwayoyin halitta;dakin gwaje-gwaje na likitanci ya ƙunshi yanki na murabba'in murabba'in mita 5400 kuma yana da ikon aiwatar da kasuwancin gano cutar kansar methylation azaman ƙwararrun dakin gwaje-gwaje na likita na ɓangare na uku.Bayan haka, muna da samfura guda uku da aka sami takardar shedar CE, waɗanda ke rufe kansar mahaifa, ciwon daji na endometrial da gano cutar kansar urothelial.
Ana iya amfani da fasahar gano ƙwayar cutar daji ta Epiprobe don gwajin cutar kansa da wuri, bincike na taimako, kimantawa kafin yin aiki da bayan aiki, sa ido kan sake dawowa, wanda ke gudana cikin dukkan hanyoyin gano cutar kansa da jiyya, yana ba da mafi kyawun mafita ga likitoci da marasa lafiya.
