TAGMe DNA Methylation Kits (qPCR) don Ciwon daji na Cervical
SIFFOFIN KIRKI
Daidaitawa
Ingantattun samfuran asibiti sama da 36000 a cikin binciken cibiyar makafi sau biyu, samfurin yana da takamaiman 94.3% da azanci na 96.0%.
Dace
Ana iya kammala ainihin fasahar gano Me-qPCR methylation a mataki ɗaya cikin sa'o'i 3 ba tare da canjin bisulfite ba.
Da wuri
Ana iya ci gaba da gwajin cutar kansar mahaifa zuwa mataki na manyan raunuka (rauni na farko).
Kayan aiki da kai
Tare da keɓantaccen software na bincike na sakamako, fassarar sakamakon an sarrafa ta atomatik kuma ana iya karantawa kai tsaye.
AMFANI DA NUFIN
Ana amfani da wannan kit ɗin don gano ingancin in vitro na hypermethylation na gene PCDHGB7 a cikin samfuran mahaifa.Sakamakon sakamako mai kyau yana nuna haɗarin haɗari na 2 ko mafi girma-sa / ƙarin ci gaba na intraepithelial neoplasia na mahaifa (CIN2 +, ciki har da CIN2, CIN3, adenocarcinoma a wuri, da kuma ciwon daji na mahaifa), wanda ke buƙatar ƙarin bincike na colposcopy da / ko histopathological jarrabawa.Akasin haka, sakamakon gwaji mara kyau yana nuna cewa haɗarin CIN2+ ya yi ƙasa kaɗan, amma haɗarin ba za a iya cire shi gaba ɗaya ba.Ya kamata ganewar asali na ƙarshe ya dogara ne akan colposcopy da/ko sakamakon histopathological.PCDHGB7 memba ne na dangin protocadherin γ tarin kwayoyin halitta.An samo Protocadherin don tsara tsarin tafiyar da ilimin halitta kamar yaduwar kwayar halitta, sake zagayowar tantanin halitta, apoptosis, mamayewa, ƙaura da autophagy na ƙwayoyin tumor ta hanyar hanyoyi daban-daban na sigina, da kuma yin shiru da kwayar cutar ta hanyar hypermethylation na yankin mai gabatarwa yana da alaka da abin da ya faru da ci gaba. na ciwon daji da yawa.An bayar da rahoton cewa hypermethylation na PCDHGB7 yana da alaƙa da ciwace-ciwace iri-iri, irin su lymphoma ba Hodgkin, ciwon nono, ciwon mahaifa, ciwon daji na endometrial da ciwon daji na mafitsara.
KA'IDAR GANO
Wannan kit ɗin ya ƙunshi reagent na hakar acid nucleic da PCR reagent.Ana fitar da acid nucleic ta hanyar tushen dutsen maganadisu.Wannan kit ɗin ya dogara ne akan ƙa'idar hanyar PCR mai ƙididdigewa, ta amfani da methylation-takamaiman halayen PCR na ainihin lokacin don nazarin DNA samfuri, kuma a lokaci guda gano wuraren CpG na PCDHGB7 gene da alamar ingancin sarrafawa ta ciki gutsuttsura kwayoyin G1 da G2.An ƙididdige matakin methylation na PCDHGB7 a cikin samfurin, ko ƙimar Me, bisa ga PCDHGB7 gene methylated DNA amplification Ct da ƙimar Ct na tunani.PCDHGB7 gene hypermethylation tabbatacce ko korau matsayi an ƙaddara bisa ga ƙimar Ni.
Yanayin aikace-aikace
Farkon nunawa
Mutane masu lafiya
Gwajin Hadarin Ciwon daji
Yawan jama'a masu haɗari (tabbatacce ga babban haɗarin ɗan adam papillomavirus (hrHPV) ko tabbatacce ga cytology exfoliation na mahaifa)
Sa ido Maimaituwa
Yawan jama'ar bayan tiyata (tare da tarihin manyan raunuka na mahaifa ko kansar mahaifa)
Muhimmancin asibiti
Binciken farko don yawan jama'a masu lafiya:Za a iya tantance cutar kansar mahaifa da kuma raunin da ya faru
Ƙimar haɗari a cikin yawan masu haɗari:Ana iya yin rarrabuwar haɗari a cikin yawan jama'a na HPV don jagorantar gano bambance-bambance na gaba
Saka idanu maimaituwa don yawan mutanen da suka biyo bayan tiyata:Ana iya yin sa ido kan sake dawowar jama'a bayan tiyata don hana jinkirin jiyya da ke haifar da sake dawowa
Tarin samfurin
Hanyar Samfur: Sanya abin da za'a iya zubar da mahaifa a cikin mahaifa os, a hankali shafa goshin mahaifa kuma a juya sau 4-5 a agogo, a hankali cire goshin mahaifa, saka shi cikin maganin adana tantanin halitta, sannan a sanya masa alama don jarrabawar ta gaba.
Ajiye samfurori:Ana iya adana samfurori a cikin zafin jiki har zuwa kwanaki 14, a 2-8 ℃ har zuwa watanni 2, kuma a -20± 5 ℃ har zuwa watanni 24.
Tsarin Ganewa: Sa'o'i 3 (Ba tare da tsari na hannu ba)
DNA Methylation Kits (qPCR) don Ciwon daji na Urothelial
| Aikace-aikacen asibiti | Binciken asibiti na asibiti na ciwon daji na mahaifa |
| Gane kwayoyin halitta | PCDHGB7 |
| Nau'in samfurin | Samfuran mahaifar mata |
| Hanyar gwaji | Fluorescence ƙididdige fasahar PCR |
| Samfurin da ya dace | Farashin ABI7500 |
| Bayanin tattarawa | 48 gwaje-gwaje/kit |
| Yanayin Ajiya | Ya kamata a adana Kit A a 2-30 ℃ Ya kamata a adana Kit B a -20± 5℃ Yana aiki har zuwa watanni 12 |
